Earthworms are predominant consumers of organic matter in terrestrial environments and as such represent a sentinel organism for detecting the presence of pharmaceuticals, and other anthropogenic wastewater indicators (AWIs) in these environments. Land application of organic rich biosolids as a fertilizer source is a common practice for disposal of the solid material produced during wastewater treatment. Biosolids are known to contain a complex mixture of a variety of AWIs including detergent metabolites, pharmaceuticals, synthetic fragrances, and more. Such chemical concentrations generally range from parts per trillion to parts per million. We show that once land applied, select AWIs can transfer from the parent biosolid material and bioaccumulate in earthworm tissue. We also observed bioaccumulation of AWIs by earthworms in controlled laboratory experiments exposed to biosolids. Earthworms were found to have quantifiable concentrations of pharmaceuticals, synthetic fragrances, detergent metabolites, disinfectants, and plasticizers. In many instances, AWIs were detected in earthworm tissue while some of these same compounds were below detection limits in the corresponding soil samples. Therefore, by virtue of bioaccumulation, earthworms may sever as a valuable tool for detecting terrestrial contamination by AWIs. Given that earthworms represent a substantial fraction of biomass in many terrestrial environments and are primary consumers of soil organic matter, they may serve as a pathway for introducing AWIs into the food chain. We will present results of AWI presence in earthworms collected from multiple agricultural sites within the United States. The sample sites represent locations of recent and historical biosolid application and therefore recent and historical AWI contamination. Results from the controlled laboratory exposure experiments indicate that the presence of biosolid can influence earthworm survival and reproduction.