Simultaneous Determination of Antibiotics in Farmed Fish

Tuesday, September 22, 2009: 2:00 p.m.
Stefan Effkemann, Ph.D. , Institute for Fish and Fishery Products, Lower Saxony Federal State Office of Consumer Protection and Food Safety (LAVES), 27472 Cuxhaven, Germany
Isabell Tolmien , Institute for Fish and Fishery Products, Lower Saxony Federal State Office of Consumer Protection and Food Safety (LAVES), 27472 Cuxhaven, Germany
Robert Kreft , Institute for Fish and Fishery Products, Lower Saxony Federal State Office of Consumer Protection and Food Safety (LAVES), 27472 Cuxhaven, Germany
Edda Bartelt , Institute for Fish and Fishery Products, Lower Saxony Federal State Office of Consumer Protection and Food Safety (LAVES), 27472 Cuxhaven, Germany
Michael Kühne , Dep. 5, Lower Saxony Federal State Office of Consumer Protection and Food Safety (LAVES), 26203 Wardenburg, Germany
A lot of the aquatic species presently in aquaculture have been domesticated since the start of the 20th century. In the last decades the price of fish began to climb, since wild fish capture rates peaked at 60 to 70 million tons per year and the human population continued to rise. In order to meet the growing demand for fish, aquaculture became more and more popular. In the next 10 to 15 years estimations predict 50-60 million tons fish per year produced in aquaculture worldwide. China is the biggest producer of fish in aquaculture with an expected total amount of 35 million tons in the year 2020.

Intensive mass animal farming often requires animal drugs. Usually antibiotics are helpful tools in order to treat bacterial infections of fish, but they are also illegally used to prevent diseases or for production purposes such as to promote growth. In the recent years many notifications in the European RAPID ALERT SYSTEM FOR FOOD AND FEED (RASFF) demonstrate the existence of a residue problem of antibiotics preferably in aquatic animals from aquaculture.

The national residue control plan aims at controlling the farms of origin, the slaughterhouses, the companies receiving the unprocessed raw products and the border checkpoints. In contrast to the broad spectrum of legal and illegal antibiotics, which can theoretically be applied, the number of substances stated in the national residue control plan seems to be comparatively low. In case of fish important compounds belonging to different substance groups e.g. quinolones, sulfonamides, macrolides, lincosamides and tetracyclines are not included yet.

In order to cover a preferably broad spectrum of drug substances a modern screening method based on LC-MS/MS was developed and applied. A method overview and detailed results will be presented.